recR [2020-04-28 18:31:48]

required for the formation of RecA DNA repair centers

Locus: BSU_00210

Isoelectric point: 5.30

Molecular weight: 21.83 kDa

Protein length: 198 aa

Gene length: 597 bp

Function: DNA repair/ recombination

Product: formation of RecA DNA repair centers

Essential: no

Synonyms: recM

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 28,867 → 29,463

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed

The protein

Catalyzed reaction/ biological activity

• required for the formation of RecA DNA repair centers (together with RecO) PubMed

Protein family

• RecR family (single member, according to UniProt)

Domains

• TOPRIM domain (aa 80-175) PubMed

Structure

• 2V1C (the RecR-RecO complex from Deinococcus radiodurans, RecR: 52% identity, 78% similarity, RecO: 30%/ 58%) PubMed

Localization

• cytoplasm (homogeneous) PubMed

Expression and Regulation

Operons

• Genes: dnaX-yaaK-recR-yaaL-bofA
  Description: PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• BKE00210 (ΔrecR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTTTTATCCCCCTA, downstream forward: _UP4_TTGTAAGGAGGAAAAAGCGA
• BKK00210 (ΔrecR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTTTTATCCCCCTA, downstream forward: _UP4_TTGTAAGGAGGAAAAAGCGA

References

Reviews

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Original publications

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